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OGD causes glutamatergic and GABAergic synapse down- regulation. To understand the mechanisms of BDNF action in ischemia, we started with an in vitro model of ischemia, OGD, in organotypic hippocampal slice cultures obtained from transgenic mice that express myristoylated enhanced green fluorescent protein ( GFP) in a subset of CA1 pyramidal neurons and studied synaptic changes in the CA1 area.

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